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KMID : 0624620100430060432
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BMB Reports
2010 Volume.43 No. 6 p.432 ~ p.437
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Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21
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Deng Yun
Li Yong-Qing
Fan Xiong-Wei
Yuan Wu-Zhou
Xie Hua-Ping
Mo Xiao-Yan
Yan Yan
Zhou Jun-Mei
Wang Yue-Qun
Ye Xian-Li
Wan Yong-Qi
Wu Xiu-Shan
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Abstract
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LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes αB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with αB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, αB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or αB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both αB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and αB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.
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KEYWORD
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LBH, p21, p53, Yeast two-hybrid, αB-crystallin
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